PDBe 4lc9

X-ray diffraction
3.4Å resolution

Structural Basis for Regulation of Human Glucokinase by Glucokinase Regulatory Protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucokinase regulatory protein Chain: A
Molecule details ›
Chain: A
Length: 636 amino acids
Theoretical weight: 70.15 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07071 (Residues: 1-627; Coverage: 100%)
Gene name: Gckr
Structure domains: Rossmann fold
Glucokinase Chain: B
Molecule details ›
Chain: B
Length: 472 amino acids
Theoretical weight: 53.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 3-465; Coverage: 100%)
  • Best match: P35557-2 (Residues: 17-466)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 95.663Å b: 105.312Å c: 132.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.292
Expression system: Escherichia coli