PDBe 4lac

X-ray diffraction
2.82Å resolution

Crystal Structure of Protein Phosphatase 2A (PP2A) and PP2A phosphatase activator (PTPA) complex with ATPgammaS


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase 2A activator Chain: B
Molecule details ›
Chain: B
Length: 308 amino acids
Theoretical weight: 35.19 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
  • Canonical: Q15257 (Residues: 19-358; Coverage: 85%)
  • Best match: Q15257-2 (Residues: 19-323)
Gene names: PPP2R4, PTPA
Sequence domains: Phosphotyrosyl phosphate activator (PTPA) protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P30153 (Residues: 366-589; Coverage: 38%)
Gene name: PPP2R1A
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 311 amino acids
Theoretical weight: 35.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 57.07Å b: 100.186Å c: 167.45Å
α: 90° β: 90° γ: 90°
R R work R free
0.191 0.188 0.242
Expression systems:
  • Trichoplusia ni
  • Escherichia coli