PDBe 4kxq

X-ray diffraction
1.85Å resolution

Structure of NAD-dependent protein deacetylase sirtuin-1 (closed state, 1.85 A)

Released:
Source organism: Homo sapiens
Primary publication:
Structural and Functional Analysis of Human SIRT1.
J. Mol. Biol. (2013)
PMID: 24120939

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SirtT1 75 kDa fragment Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 31.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96EB6 (Residues: 234-510; Coverage: 37%)
Gene names: SIR2L1, SIRT1
Sequence domains: Sir2 family
Structure domains:
NAD-dependent protein deacetylase sirtuin-1 Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96EB6 (Residues: 641-663; Coverage: 3%)
Gene names: SIR2L1, SIRT1

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 92.714Å b: 92.714Å c: 97.745Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.189
Expression system: Escherichia coli BL21(DE3)