PDBe 4ksl

X-ray diffraction
2.83Å resolution

Gumby/Fam105B in complex with linear di-ubiquitin

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin thioesterase otulin Chains: A, B, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, B, E, G, I, K, M, O, Q, S, U, W
Length: 276 amino acids
Theoretical weight: 32.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96BN8 (Residues: 79-352; Coverage: 78%)
Gene names: FAM105B, OTULIN
Sequence domains: Peptidase family C101
Ubiquitin Chains: C, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: C, D, F, H, J, L, N, P, R, T, V, X
Length: 156 amino acids
Theoretical weight: 17.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 532-684; Coverage: 22%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 183.622Å b: 186.087Å c: 219.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.254
Expression system: Escherichia coli BL21(DE3)