PDBe 4kro

X-ray diffraction
3.05Å resolution

Nanobody/VHH domain EgA1 in complex with the extracellular region of EGFR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cetuximab heavy chain Chain: D
Molecule details ›
Chain: D
Length: 220 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Cetuximab light chain Chain: C
Molecule details ›
Chain: C
Length: 211 amino acids
Theoretical weight: 23.16 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Nanobody/VHH domain EgA1 Chain: B
Molecule details ›
Chain: B
Length: 138 amino acids
Theoretical weight: 15.55 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 69.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 25-642; Coverage: 52%)
  • Best match: P00533-4 (Residues: 25-627)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 66.207Å b: 96.288Å c: 128.097Å
α: 90° β: 100.68° γ: 90°
R-values:
R R work R free
0.224 0.22 0.28
Expression systems:
  • Mus musculus
  • Escherichia coli
  • Spodoptera frugiperda