PDBe 4krl

X-ray diffraction
2.85Å resolution

Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 6.0

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nanobody/VHH domain 7D12 Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 14.58 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 335-538; Coverage: 17%)
  • Best match: P00533-2 (Residues: 335-404)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Receptor L domain
Structure domains: 24 nucleotide stem-loop, u2 snrnp hairpin iv. U2 a'. Chain A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6122
Unit cell:
a: 148.048Å b: 148.048Å c: 82.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.268
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda