PDBe 4ki0

X-ray diffraction
2.38Å resolution

Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose/maltodextrin import ATP-binding protein MalK Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 42.18 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P68187 (Residues: 1-371; Coverage: 100%)
Gene names: JW3995, b4035, malK
Sequence domains:
Structure domains:
Maltose-binding periplasmic protein Chain: E
Molecule details ›
Chain: E
Length: 380 amino acids
Theoretical weight: 41.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II
Maltose transport system permease protein MalF Chain: F
Molecule details ›
Chain: F
Length: 514 amino acids
Theoretical weight: 57.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02916 (Residues: 1-514; Coverage: 100%)
Gene names: JW3993, b4033, malF
Sequence domains:
Structure domains:
Maltose transport system permease protein MalG Chain: G
Molecule details ›
Chain: G
Length: 296 amino acids
Theoretical weight: 32.25 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P68183 (Residues: 1-296; Coverage: 100%)
Gene names: JW3992, b4032, malG
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 81.592Å b: 96.631Å c: 112.321Å
α: 85.3° β: 79.11° γ: 73°
R-values:
R R work R free
0.194 0.192 0.228
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)