PDBe 4k94

X-ray diffraction
2.4Å resolution

Crystal structure of KIT D4D5 fragment in complex with anti-Kit antibody Fab19

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab19 heavy chain Chain: L
Molecule details ›
Chain: L
Length: 226 amino acids
Theoretical weight: 24.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Fab19 light chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Mast/stem cell growth factor receptor Kit Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 24.04 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P10721 (Residues: 308-518; Coverage: 22%)
Gene names: KIT, SCFR
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 169.58Å b: 48.98Å c: 106.97Å
α: 90° β: 122.38° γ: 90°
R-values:
R R work R free
0.2 0.198 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • unidentified baculovirus