PDBe 4k48

X-ray diffraction
2.49Å resolution

Structure of the Streptococcus pneumoniae leucyl-tRNA synthetase editing domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.09 KDa
Source organism: Streptococcus pneumoniae ATCC 700669
Expression system: Escherichia coli
UniProt:
  • Canonical: B8ZKS5 (Residues: 228-410; Coverage: 22%)
Gene names: SPN23F02440, leuS
Structure domains: Isoleucyl-tRNA Synthetase; Domain 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 88.018Å b: 88.018Å c: 135.405Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.241
Expression system: Escherichia coli