PDBe 4jxt

X-ray diffraction
1.9Å resolution

CID of human RPRD1A in complex with a phosphorylated peptide from RPB1-CTD

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulation of nuclear pre-mRNA domain-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 16.06 KDa
Source organism: Homo sapiens
Expression system: Acidovorax soli
UniProt:
  • Canonical: Q96P16 (Residues: 1-137; Coverage: 44%)
Gene names: P15RS, RPRD1A
Sequence domains: RNA polymerase II-binding domain.
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
DNA-directed RNA polymerase II subunit RPB1 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1786-1805; Coverage: 1%)
Gene names: POLR2, POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: I4
Unit cell:
a: 93.264Å b: 93.264Å c: 36.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.237
Expression systems:
  • Acidovorax soli
  • Not provided