PDBe 4jxf

X-ray diffraction
2.4Å resolution

Crystal Structure of PLK4 Kinase with an inhibitor: 400631 ((1R,2S)-2-{3-[(E)-2-{4-[(DIMETHYLAMINO)METHYL]PHENYL}ETHENYL]-2H-INDAZOL-6-YL}-5'-METHOXYSPIRO[CYCLOPROPANE-1,3'-INDOL]-2'(1'H)-ONE)

Released:
Source organism: Homo sapiens
Entry authors: Qiu W, Plotnikova O, Feher M, Awrey DE, Chirgadze NY

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK4 Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00444 (Residues: 4-269; Coverage: 27%)
Gene names: PLK4, SAK, STK18
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I23
Unit cell:
a: 127.571Å b: 127.571Å c: 127.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.254 0.268
Expression system: Escherichia coli