PDBe 4jqw

X-ray diffraction
2.9Å resolution

Crystal Structure of a Complex of NOD1 CARD and Ubiquitin

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of a complex of NOD1 CARD and ubiquitin.
OpenAccess logo PLoS ONE 9 e104017 (2014)
PMID: 25127239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleotide-binding oligomerization domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y239 (Residues: 16-108; Coverage: 10%)
Gene names: CARD4, NOD1
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas
Ubiquitin Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P41212
Unit cell:
a: 61.688Å b: 61.688Å c: 86.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.27
Expression system: Escherichia coli