PDBe 4jn4

X-ray diffraction
2.3Å resolution

Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP


Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Chaperone protein DnaK Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 65.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0A6Y8 (Residues: 2-610; Coverage: 95%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I422
Unit cell:
a: 291.739Å b: 291.739Å c: 99.5Å
α: 90° β: 90° γ: 90°
R R work R free
0.164 0.162 0.197
Expression system: Escherichia coli BL21(DE3)