PDBe 4jio

X-ray diffraction
3.6Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BRO1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 39.61 KDa
Source organism: Naumovozyma castellii CBS 4309
Expression system: Escherichia coli
UniProt:
  • Canonical: G0VBG6 (Residues: 370-708; Coverage: 39%)
Gene names: NCAS0B02080, NCAS_0B02080
Sequence domains: ALIX V-shaped domain binding to HIV
Structure domains:
Ubiquitin Chain: U
Molecule details ›
Chain: U
Length: 76 amino acids
Theoretical weight: 8.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 63.43Å b: 92.32Å c: 229.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.372 0.366 0.421
Expression system: Escherichia coli