PDBe 4jfo

X-ray diffraction
2.11Å resolution

A2 HLA complex with E1A heteroclitic variant of Melanoma peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
E1A heteroclitic Melanoma peptide Chains: C, F
Molecule details ›
Chains: C, F
Length: 10 amino acids
Theoretical weight: 927 Da
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 1-BM-C
Spacegroup: P1
Unit cell:
a: 50.283Å b: 63.255Å c: 75.098Å
α: 81.96° β: 76.09° γ: 77.98°
R-values:
R R work R free
0.222 0.219 0.296
Expression system: Escherichia coli