PDBe 4jbw

X-ray diffraction
3.91Å resolution

Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O + maltose(Out) = ADP + phosphate + maltose(In). 
[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L- histidine + D-glucose 6-phosphate(Side 2). 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose transport system permease protein MalF Chains: F, H
Molecule details ›
Chains: F, H
Length: 514 amino acids
Theoretical weight: 57.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02916 (Residues: 1-514; Coverage: 100%)
Gene names: JW3993, b4033, malF
Sequence domains:
Structure domains:
Maltose transport system permease protein MalG Chains: G, I
Molecule details ›
Chains: G, I
Length: 296 amino acids
Theoretical weight: 32.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68183 (Residues: 1-296; Coverage: 100%)
Gene names: JW3992, b4032, malG
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Maltose/maltodextrin import ATP-binding protein MalK Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 42.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68187 (Residues: 1-371; Coverage: 100%)
Gene names: JW3995, b4035, malK
Sequence domains:
Structure domains:
PTS system glucose-specific EIIA component Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 172 amino acids
Theoretical weight: 18.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69783 (Residues: 1-169; Coverage: 100%)
Gene names: JW2410, b2417, crr, gsr, iex, tgs, treD
Sequence domains: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1
Structure domains: Glucose Permease (Domain IIA)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 94.529Å b: 208.448Å c: 347.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.281
Expression system: Escherichia coli