PDBe 4j9e

X-ray diffraction
1.4Å resolution

Crystal structure of the N114A mutant of the Abl-SH3 domain complexed with the high affinity peptide P17

Released:
Source organism: Homo sapiens
Entry author: Camara-Artigas A

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 63 amino acids
Theoretical weight: 6.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 60-121; Coverage: 6%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH3 domain
Structure domains: SH3 Domains
P17 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 11 amino acids
Theoretical weight: 1.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 86.23Å b: 86.23Å c: 45.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.192
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided