› 4j7a

X-ray diffraction
1.49Å resolution

Crystal Structure of Est25 - a Bacterial Homolog of Hormone-Sensitive Lipase from a Metagenomic Library

Released:
DOI: 10.2210/pdb4j7a/pdb
PDB entry 4j7a coloured by chain, front view.
PDB entry 4j7a coloured by chain, side view.
PDB entry 4j7a coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural and functional analyses of a bacterial homologue of hormone-sensitive lipase from a metagenomic library.
Ngo TD, Ryu BH, Ju H, Jang E, Park K, Kim KK, Kim TD
Acta Crystallogr D Biol Crystallogr 69 1726-37 (2013)
PMID: 23999296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 374 amino acids
Theoretical weight: 39.74 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4TZQ3 (Residues: 1-362; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 197.758Å b: 95.213Å c: 99.418Å
α: 90° β: 97.05° γ: 90°
R-values:
R R work R free
0.15 0.143 0.17
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family.
Li et al. (2015)
11 more

10 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
9 more