PDBe 4iza

X-ray diffraction
1.93Å resolution

Structure of Dually Phosphorylated ERK2 bound to the PEA-15 Death Effector Domain

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 356 amino acids
Theoretical weight: 41.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P28482 (Residues: 8-360; Coverage: 98%)
Gene names: ERK2, MAPK1, PRKM1, PRKM2
Sequence domains: Protein kinase domain
Structure domains:
Astrocytic phosphoprotein PEA-15 Chain: B
Molecule details ›
Chain: B
Length: 97 amino acids
Theoretical weight: 11.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q15121 (Residues: 1-96; Coverage: 74%)
Gene name: PEA15
Sequence domains: Death effector domain
Structure domains: Death Domain, Fas

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 73.758Å b: 204.024Å c: 61.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.243
Expression system: Escherichia coli BL21(DE3)