PDBe 4ium

X-ray diffraction
1.45Å resolution

Equine arteritis virus papain-like protease 2 (PLP2) covalently bound to ubiquitin

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nsp2 cysteine proteinase Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.23 KDa
Source organism: Equine arteritis virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19811 (Residues: 261-392; Coverage: 4%)
Gene names: 1a-1b, rep
Sequence domains: Equine arterivirus Nsp2-type cysteine proteinase
Structure domains: Cathepsin B; Chain A
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-683; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 38.32Å b: 62.04Å c: 84.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.178
Expression system: Escherichia coli