PDBe 4isg

X-ray diffraction
2.64Å resolution

Human glucokinase in complex with novel activator (2S)-3-cyclohexyl-2-[4-(methylsulfonyl)-2-oxopiperazin-1-yl]-N-(1,3-thiazol-2-yl)propanamide


Function and Biology Details

Reaction catalysed:
ATP + D-glucose = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glucokinase Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
  • Canonical: P35557 (Residues: 16-465; Coverage: 97%)
  • Best match: P35557-2 (Residues: 17-466)
Gene name: GCK
Sequence domains: Hexokinase
Structure domains: Hexokinase; domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 49.256Å b: 78.26Å c: 120.261Å
α: 90° β: 90° γ: 90°
R R work R free
0.203 0.199 0.288
Expression system: Escherichia coli DH5[alpha]