PDBe 4is0

X-ray diffraction
1.72Å resolution

Structural Insights into Omega-Class Glutathione Transferases: A Snapshot of Enzyme Reduction and Identification of the Non-Catalytic Ligandin Site.

Released:

Function and Biology Details

Reactions catalysed:
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase omega-1 Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78417 (Residues: 1-241; Coverage: 100%)
Gene names: GSTO1, GSTTLP28
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand 1R4 1 x 1R4

Cofactor: Ligand GDS 1 x GDS
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL4-2
Spacegroup: P3121
Unit cell:
a: 57.6Å b: 57.6Å c: 140.176Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.152 0.201
Expression system: Escherichia coli BL21(DE3)