PDBe 4ir8

X-ray diffraction
1.85Å resolution

1.85 Angstrom Crystal Structure of Putative Sedoheptulose-1,7 bisphosphatase from Toxoplasma gondii

Released:
Source organism: Toxoplasma gondii ME49
Entry authors: Minasov G, Ruan J, Wawrzak Z, Halavaty A, Shuvalova L, Harb OS, Ngo H, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Sedoheptulose 1,7-bisphosphate + H(2)O = sedoheptulose 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sedoheptulose-1,7 bisphosphatase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.85 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9PW60 (Residues: 1-330; Coverage: 100%)
Gene names: BN1205_061570, TGVEG_235700
Sequence domains: Fructose-1-6-bisphosphatase, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P6522
Unit cell:
a: 64.4Å b: 64.4Å c: 591.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.185
Expression system: Escherichia coli BL21(DE3)