PDBe 4i5l

X-ray diffraction
2.43Å resolution

Structural mechanism of trimeric PP2A holoenzyme involving PR70: insight for Cdc6 dephosphorylation


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, D
Molecule details ›
Chains: A, D
Length: 584 amino acids
Theoretical weight: 64.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P30153 (Residues: 6-589; Coverage: 99%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Cell division control protein 6 homolog; Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta Chains: B, E
Molecule details ›
Chains: B, E
Length: 413 amino acids
Theoretical weight: 47.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q99741 (Residues: 70-90; Coverage: 4%)
  • Canonical: Q9Y5P8 (Residues: 120-490; Coverage: 65%)
  • nullnull
Gene names: CDC18L, CDC6, PPP2R3B, PPP2R3L
Sequence domains:
Structure domains:
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 311 amino acids
Theoretical weight: 35.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Microcystin-LR (MCLR) bound form Chains: G, H
Molecule details ›
Chains: G, H
Length: 7 amino acids
Theoretical weight: 1.02 KDa
Source organism: Microcystis aeruginosa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 94.234Å b: 101.071Å c: 343.396Å
α: 90° β: 90° γ: 90°
R R work R free
0.181 0.179 0.228
Expression systems:
  • Escherichia coli
  • Trichoplusia ni