› 4i5i

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SirtT1 75 kDa fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96EB6 (Residues: 241-516; Coverage: 37%)
Gene names: SIR2L1, SIRT1
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 4I5 2 x 4I5
Ligand NAD 2 x NAD
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P32
Unit cell:
a: 106.513Å b: 106.513Å c: 80.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.195
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Sirtuin activators and inhibitors: Promises, achievements, and challenges.
Dai et al. (2018)
11 more

51 mentions without citation

Lysine Acetylation Goes Global: From Epigenetics to Metabolism and Therapeutics.
Ali et al. (2018)
50 more