PDBe 4i21

X-ray diffraction
3.37Å resolution

Crystal structure of L858R + T790M EGFR kinase domain in complex with MIG6 peptide


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P00533 (Residues: 695-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase
Structure domains:
ERBB receptor feedback inhibitor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 62 amino acids
Theoretical weight: 6.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9UJM3 (Residues: 315-374; Coverage: 13%)
Gene names: ERRFI1, MIG6
Sequence domains: EGFR receptor inhibitor Mig-6

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 38.666Å b: 66.05Å c: 94.518Å
α: 71.85° β: 87.38° γ: 74.26°
R R work R free
0.233 0.23 0.279
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli