PDBe 4hlk

X-ray diffraction
2Å resolution

Crystal structure of Tankyrase 2 in complex with 4'-methylflavone

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly [ADP-ribose] polymerase tankyrase-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H2K2 (Residues: 946-1113; Coverage: 14%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3
Poly [ADP-ribose] polymerase tankyrase-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 49 amino acids
Theoretical weight: 5.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H2K2 (Residues: 1114-1162; Coverage: 4%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Structure domains: YefM-like fold

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 93.97Å b: 95.57Å c: 118.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.215
Expression system: Escherichia coli BL21(DE3)