PDBe 4hfz

X-ray diffraction
2.69Å resolution

Crystal Structure of an MDM2/P53 Peptide Complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 109 amino acids
Theoretical weight: 12.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00987 (Residues: 17-125; Coverage: 22%)
  • Best match: Q00987-6 (Residues: 17-29)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain
Cellular tumor antigen p53 Chains: B, D
Molecule details ›
Chains: B, D
Length: 15 amino acids
Theoretical weight: 1.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04637 (Residues: 15-29; Coverage: 4%)
Gene names: P53, TP53

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 52.944Å b: 52.944Å c: 196.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided