PDBe 4he8

X-ray diffraction
3.3Å resolution

Crystal structure of the membrane domain of respiratory complex I from Thermus thermophilus

Released:
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of the entire respiratory complex I.
OpenAccess logo Nature 494 443-8 (2013)
PMID: 23417064

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
NADH-quinone oxidoreductase subunit 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.15 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56217 (Residues: 1-119; Coverage: 100%)
Gene names: TTHA0084, nqo7
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
NADH-quinone oxidoreductase subunit 10 Chains: D, J
Molecule details ›
Chains: D, J
Length: 176 amino acids
Theoretical weight: 18.56 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56225 (Residues: 1-176; Coverage: 100%)
Gene names: TTHA0093, nqo10
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
NADH-quinone oxidoreductase subunit 11 Chains: E, K
Molecule details ›
Chains: E, K
Length: 95 amino acids
Theoretical weight: 10 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56226 (Residues: 1-95; Coverage: 100%)
Gene names: TTHA0094, nqo11
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-quinone oxidoreductase subunit 12 Chains: F, L
Molecule details ›
Chains: F, L
Length: 606 amino acids
Theoretical weight: 65.19 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56227 (Residues: 1-606; Coverage: 100%)
Gene names: TTHA0095, nqo12
Sequence domains:
NADH-quinone oxidoreductase subunit 13 Chains: G, M
Molecule details ›
Chains: G, M
Length: 469 amino acids
Theoretical weight: 49.25 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56228 (Residues: 1-469; Coverage: 100%)
Gene names: TTHA0096, nqo13
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit 14 Chains: I, N
Molecule details ›
Chains: I, N
Length: 427 amino acids
Theoretical weight: 44.46 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56229 (Residues: 1-427; Coverage: 100%)
Gene names: TTHA0097, nqo14
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit 8 Chains: C, H
Molecule details ›
Chains: C, H
Length: 365 amino acids
Theoretical weight: 41.03 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q60019 (Residues: 1-365; Coverage: 100%)
Gene names: TTHA0091, nqo8
Sequence domains: NADH dehydrogenase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 85.26Å b: 120.54Å c: 176.66Å
α: 91.91° β: 95.73° γ: 101.41°
R-values:
R R work R free
0.21 0.209 0.263