PDBe 4hd8

X-ray diffraction
2.3Å resolution

Crystal structure of human Sirt3 in complex with Fluor-de-Lys peptide and piceatannol

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NTG7 (Residues: 116-399; Coverage: 71%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:
Fluor-de-Lys peptide Chain: F
Molecule details ›
Chain: F
Length: 4 amino acids
Theoretical weight: 770 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: R32
Unit cell:
a: 114.6Å b: 114.6Å c: 123.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided