PDBe 4hcp

X-ray diffraction
2.52Å resolution

crystal structure of Burkholderia pseudomallei effector protein chbp in complex with nedd8

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative ATP/GTP binding protein Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.56 KDa
Source organism: Burkholderia pseudomallei K96243
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63KH5 (Residues: 78-328; Coverage: 77%)
Gene name: BPSS1385
Sequence domains: Cycle inhibiting factor (CIF)
NEDD8 Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15843 (Residues: 1-76; Coverage: 94%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 127.389Å b: 44.973Å c: 67.303Å
α: 90° β: 110.83° γ: 90°
R-values:
R R work R free
0.203 0.2 0.267
Expression system: Escherichia coli BL21(DE3)