PDBe 4h6j

X-ray diffraction
1.52Å resolution

Identification of Cys 255 in HIF-1 as a novel site for development of covalent inhibitors of HIF-1 /ARNT PasB domain protein-protein interaction.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q16665 (Residues: 238-348; Coverage: 13%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8
Sequence domains: PAS fold
Structure domains: Beta-Lactamase
Aryl hydrocarbon receptor nuclear translocator Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P27540 (Residues: 357-470; Coverage: 14%)
Gene names: ARNT, BHLHE2
Sequence domains: PAS domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 68.841Å b: 44.94Å c: 78.087Å
α: 90° β: 96.54° γ: 90°
R-values:
R R work R free
0.216 0.215 0.248
Expression system: Escherichia coli BL21