PDBe 4grw

X-ray diffraction
2.55Å resolution

Structure of a complex of human IL-23 with 3 Nanobodies (Llama vHHs)

Released:
Source organisms:
Entry authors: Desmyter A, Spinelli S, Button C, Saunders M, de Haard H, Rommelaere H, Union A, Cambillau C

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Nanobody 37D5 Chains: F, J
Molecule details ›
Chains: F, J
Length: 126 amino acids
Theoretical weight: 13.85 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Nanobody 124C4 Chains: E, G
Molecule details ›
Chains: E, G
Length: 125 amino acids
Theoretical weight: 13.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Nanobody 22E11 Chains: H, I
Molecule details ›
Chains: H, I
Length: 123 amino acids
Theoretical weight: 13.38 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Interleukin-12 subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 328 amino acids
Theoretical weight: 37.21 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29460 (Residues: 1-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-23 subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 189 amino acids
Theoretical weight: 20.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NPF7 (Residues: 1-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 102.27Å b: 135.14Å c: 139.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.218
Expression systems:
  • Escherichia coli BL21
  • Spodoptera frugiperda