› 4gpz

X-ray diffraction
1.65Å resolution

Crystal structure of human B type phosphoglycerate mutase H11 phosphorylated form

Released:
DOI: 10.2210/pdb4gpz/pdb
PDB entry 4gpz coloured by chain, front view.
PDB entry 4gpz coloured by chain, side view.
PDB entry 4gpz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tyr26 phosphorylation of PGAM1 provides a metabolic advantage to tumours by stabilizing the active conformation.
Hitosugi T, Zhou L, Fan J, Elf S, Zhang L, Xie J, Wang Y, Gu TL, Alečković M, LeRoy G, Kang Y, Kang HB, Seo JH, Shan C, Jin P, Gong W, Lonial S, Arellano ML, Khoury HJ, Chen GZ, Shin DM, Khuri FR, Boggon TJ, Kang S, He C, Chen J
Nat Commun 4 1790 (2013)
PMID: 23653202

Function and Biology Details

Reactions catalysed: 
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate mutase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18669 (Residues: 1-254; Coverage: 100%)
Gene names: CDABP0006, PGAM1, PGAMA
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MES 2 x MES
1 modified residue:
Ligand NEP 2 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 77.36Å b: 81.174Å c: 90.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

17 review citations

6-Phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1-AMPK signalling.
Lin et al. (2015)
16 more

5 mentions without citation

Tyr26 phosphorylation of PGAM1 provides a metabolic advantage to tumours by stabilizing the active conformation.
Hitosugi et al. (2013)
4 more