PDBe 4gnk

X-ray diffraction
4Å resolution

Crystal structure of Galphaq in complex with full-length human PLCbeta3

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(q) subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 353 amino acids
Theoretical weight: 41.54 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P21279 (Residues: 7-359; Coverage: 98%)
Gene name: Gnaq
Sequence domains: G-protein alpha subunit
Structure domains:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1235 amino acids
Theoretical weight: 139.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q01970 (Residues: 10-1234; Coverage: 99%)
Gene name: PLCB3
Sequence domains:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 30.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q01970 (Residues: 934-1192; Coverage: 21%)
Gene name: PLCB3
Structure domains: Phospholipase C Beta; Chain: A

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D, APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 91.423Å b: 188.845Å c: 293.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.255
Expression system: Trichoplusia ni