PDBe 4gne

X-ray diffraction
1.47Å resolution

Crystal Structure of NSD3 tandem PHD5-C5HCH domains complexed with H3 peptide 1-7

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase NSD3 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZ95 (Residues: 1310-1413; Coverage: 7%)
Gene names: DC28, NSD3, WHSC1L1
Sequence domains: NSD Cys-His rich domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.3 Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 777 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 2-8; Coverage: 5%)
Gene names: H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6522
Unit cell:
a: 57.2Å b: 57.2Å c: 107.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.148 0.178
Expression systems:
  • Escherichia coli
  • Not provided