PDBe 4gfu

X-ray diffraction
2Å resolution

PTPN18 in complex with HER2-pY1248 phosphor-peptides

Released:
Source organism: Homo sapiens
Entry authors: Wang HM, Yang F, Du YJ, Yang DX, Zhang Y, Yu X, Sun JP

Function and Biology Details

Reactions catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor tyrosine-protein kinase erbB-2 Chain: F
Molecule details ›
Chain: F
Length: 7 amino acids
Theoretical weight: 886 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04626 (Residues: 1246-1252; Coverage: 1%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Tyrosine-protein phosphatase non-receptor type 18 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99952 (Residues: 6-300; Coverage: 64%)
  • Best match: Q99952-2 (Residues: 32-193)
Gene names: BDP1, PTPN18
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U, BSRF BEAMLINE 3W1A
Spacegroup: P21
Unit cell:
a: 44.032Å b: 71.766Å c: 57.161Å
α: 90° β: 99.52° γ: 90°
R-values:
R R work R free
0.192 0.189 0.232
Expression systems:
  • Not provided
  • Escherichia coli BL21