PDBe 4gdf

X-ray diffraction
2.8Å resolution

A Crystal Structure of SV40 Large T Antigen

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 497 amino acids
Theoretical weight: 57.45 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03070 (Residues: 131-627; Coverage: 70%)
Sequence domains: Origin of replication binding protein
Structure domains:
DNA (32-MER) Chains: C, G
Molecule details ›
Chains: C, G
Length: 32 nucleotides
Theoretical weight: 9.89 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (32-MER) Chains: D, H
Molecule details ›
Chains: D, H
Length: 32 nucleotides
Theoretical weight: 9.79 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 73.633Å b: 128.307Å c: 166.12Å
α: 90° β: 89.91° γ: 90°
R-values:
R R work R free
0.227 0.227 0.255
Expression systems:
  • Escherichia coli
  • Not provided