PDBe 4g6l

X-ray diffraction
2.7Å resolution

Crystal structure of human CDK8/CYCC in the DMG-in conformation

Released:
Source organism: Homo sapiens
Primary publication:
Structure-kinetic relationship study of CDK8/CycC specific compounds.
Proc. Natl. Acad. Sci. U.S.A. 110 8081-6 (2013)
PMID: 23630251

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 47.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P49336 (Residues: 1-403; Coverage: 87%)
Gene name: CDK8
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-C Chain: B
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 33.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P24863 (Residues: 1-283; Coverage: 100%)
Gene name: CCNC
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 70.181Å b: 70.942Å c: 170.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.266
Expression system: Spodoptera frugiperda