PDBe 4g1c

X-ray diffraction
1.94Å resolution

Human SIRT5 bound to Succ-IDH2 and Carba-NAD

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacylase sirtuin-5, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NXA8 (Residues: 36-302; Coverage: 86%)
Gene names: SIR2L5, SIRT5
Sequence domains: Sir2 family
Structure domains:
Succinylated IDH2 peptide Chains: D, E
Molecule details ›
Chains: D, E
Length: 7 amino acids
Theoretical weight: 615 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 67.239Å b: 84.425Å c: 95.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.242
Expression systems:
  • Escherichia coli
  • Not provided