PDBe 4fvt

X-ray diffraction
2.47Å resolution

Human SIRT3 bound to Ac-ACS peptide and Carba-NAD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NTG7 (Residues: 122-395; Coverage: 69%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:
Acetylated ACS2 peptide Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 545 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 77.43Å b: 131.05Å c: 77.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.249
Expression systems:
  • Escherichia coli
  • Not provided