PDBe 4ftv

X-ray diffraction
2.74Å resolution

The complex between the high affinity version of A6 TCR (A6c134) and human Class I MHC HLA-A2 with the bound TAX nonameric peptide

Released:
Entry authors: Borbulevych OY, Baker BM

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
A6 beta chain Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 27.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Protein Tax-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell lymphotropic virus type 1 (african isolate)
Expression system: Not provided
UniProt:
  • Canonical: P0C213 (Residues: 11-19; Coverage: 3%)
Gene name: tax
A6 alpha chain Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 224.23Å b: 48.954Å c: 94.398Å
α: 90° β: 89.62° γ: 90°
R-values:
R R work R free
0.218 0.216 0.26
Expression systems:
  • Escherichia coli BL21
  • Not provided