PDBe 4fm2

X-ray diffraction
2.9Å resolution

Pyrococcus abyssi B family DNA polymerase (triple mutant) bound to a dsDNA, in edition mode

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase 1 Chain: A
Molecule details ›
Chain: A
Length: 793 amino acids
Theoretical weight: 91.66 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL77 (Residues: 1-771; Coverage: 100%)
Gene names: PAB1128, PYRAB17200, pol, polI
Sequence domains:
Structure domains:
Template strand Chain: T
Molecule details ›
Chain: T
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Not provided
Primer strand Chain: P
Molecule details ›
Chain: P
Length: 8 nucleotides
Theoretical weight: 2.41 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 68.73Å b: 115.02Å c: 127.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.222
Expression systems:
  • Escherichia coli
  • Not provided