PDBe 4fbn

X-ray diffraction
2.4Å resolution

Insights into structural integration of the PLCgamma regulatory region and mechanism of autoinhibition and activation based on key roles of SH2 domains

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19174 (Residues: 545-790; Coverage: 19%)
Gene names: PLC1, PLCG1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 53.752Å b: 59.61Å c: 76.923Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.295
Expression system: Escherichia coli