PDBe 4f5b

X-ray diffraction
1.57Å resolution

Triple mutant Src SH2 domain bound to phosphotyrosine

Source organism: Homo sapiens
Primary publication:
Superbinder SH2 Domains Act as Antagonists of Cell Signaling.
Sci Signal 5 ra68 (2012)
PMID: 23012655

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P12931 (Residues: 144-252; Coverage: 20%)
Gene names: SRC, SRC1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P61
Unit cell:
a: 67.6Å b: 67.6Å c: 46.8Å
α: 90° β: 90° γ: 120°
R R work R free
0.2 0.198 0.23
Expression system: Escherichia coli BL21(DE3)