PDBe 4ehp

X-ray diffraction
2.66Å resolution

Crystal Structure of human vinculin head domain (residues 1-252) in complex with alpha-catenin (residues 277-382)

Released:
Source organism: Homo sapiens
Primary publication:
The cytoskeletal protein α-catenin unfurls upon binding to vinculin.
J. Biol. Chem. 287 18492-9 (2012)
PMID: 22493458

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18206 (Residues: 1-252; Coverage: 22%)
Gene name: VCL
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
Catenin alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 12.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35221 (Residues: 277-382; Coverage: 12%)
Gene name: CTNNA1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 57.25Å b: 73.96Å c: 107.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.192 0.229
Expression system: Escherichia coli BL21(DE3)