PDBe 4e7r

X-ray diffraction
2.25Å resolution

Thrombin in complex with 3-amidinophenylalanine inhibitor


Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chains: G, H
Molecule details ›
Chains: G, H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin variant-2 Chains: I, J
Molecule details ›
Chains: I, J
Length: 11 amino acids
Theoretical weight: 1.49 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
  • Canonical: P09945 (Residues: 62-72; Coverage: 17%)

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P1
Unit cell:
a: 50.242Å b: 50.284Å c: 71.837Å
α: 97.61° β: 96.61° γ: 90.57°
R R work R free
0.174 0.171 0.223
Expression system: Not provided