› 4dz0

X-ray diffraction
2.5Å resolution

Crystal structure of the Cu-adduct of human H-Ferritin variant MIC1 labeled with a dansyl fluorophore

Released:
DOI: 10.2210/pdb4dz0/pdb
PDB entry 4dz0 coloured by chain, front view.
PDB entry 4dz0 coloured by chain, side view.
PDB entry 4dz0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Re-engineering protein interfaces yields copper-inducible ferritin cage assembly.
Huard DJ, Kane KM, Tezcan FA
Nat Chem Biol 9 169-76 (2013)
PMID: 23340339

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 20.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 6-177; Coverage: 94%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand AEN 1 x AEN
Ligand CU 3 x CU
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: F432
Unit cell:
a: 180.434Å b: 180.434Å c: 180.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.224 0.3
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
16 more

2 mentions without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)
1 more