PDBe 4d9r

X-ray diffraction
2.42Å resolution

Inhibiting Alternative Pathway Complement Activation by Targeting the Exosite on Factor D

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Complement factor D Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00746 (Residues: 26-253; Coverage: 98%)
Gene names: CFD, DF, PFD
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Fab light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 214 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab heavy chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 218 amino acids
Theoretical weight: 23.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 132.048Å b: 132.048Å c: 180.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.251
Expression system: Escherichia coli