PDBe 4d4k

X-ray diffraction
3.24Å resolution

human PFKFB3 in complex with a pyrrolopyrimidone compound

Released:
Source organism: Homo sapiens
Entry authors: Stgallay SA, Bennett N, Critchlow S, Davies G, Debreczeni JE, Evans N, Holdgate G, Jones NP, Leach L, Maman S, McLoughlin S, Preston M, Rigoreau L, Thomas A, Walker G, Walsch J, Ward RA, Wheatley E, Winter J

Function and Biology Details

Reactions catalysed:
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 52.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q16875 (Residues: 1-449; Coverage: 86%)
Gene name: PFKFB3
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6522
Unit cell:
a: 102.97Å b: 102.97Å c: 256.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.21
Expression system: Escherichia coli BL21